Virus:Article Title: Biochemical Mechanisms and Catabolic Enzymes Involved in Bacterial Estrogen Degradation Pathways.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains Sphingomonas sp. strain KC8 Yu et al., 2007 N/A Escherichia coli BL21 (DE3) Yeastern Biotech Cat# FYE207-40VL Escherichia coli One Shot TOP10 Invitrogen Cat# C404010 Chemicals, Peptides, and Recombinant Proteins 17b-Estradiol (E2) Sigma-Aldrich Cat# E8875 Estrone (E1) Sigma-Aldrich Cat# E9750 4-Hydroxyestrone Sigma-Aldrich Cat# H6012 Dimethyl sulfoxide Sigma-Aldrich Cat# 34943 Testosterone Sigma-Aldrich Cat# 86500 Oxygen-18O2 (97 atom %) Sigma-Aldrich Cat# 490474 Estrone (3,4-13C2, 99%) Cambridge Isotope Laboratories Cat# CLM-673 Testosterone (2,3,4-13C3, 98%) Isosciences Cat# 6066 Pyridinestrone acid This paper N/A OecAhis This paper N/A OecChis This paper N/A Critical Commercial Assays XenoScreen YES/YAS Assay Xenometrix Cat# NO5-230-P In-Fusion HD Cloning Kit Clontech Cat# 639649 pEXP-5-NT/TOPO TA Expression Kit Invitrogen Cat# V96005 Deposited Data Genome sequence of Spingomonas sp. strain KC8 This paper NCBI: CP016306 Oligonucleotides pET21b-oecA forward primer: 50-GAAGGAGATATACATATGGATGGCGATCAATCG-30 This paper N/A pET21b-oecA reverse primer: 50-GTGGTGGTGCTCGAGGCCCTGCTTCGGCG-30 This paper N/A oecC forward primer (oecC_for): 50-TTGAAGATCTGCGGCTTGGGCT-30 This paper N/A oecC reverse primer (oecC_rev): 50-TCAGCCTGCGGCCACATCGTCGGC-30 This paper N/A Recombinant DNA pET21b Novagen Cat# 69741 pET21b-oecA This paper N/A pEXP-5-NT/TOPO-oecC This paper N/A Software and Algorithms MassLynx Mass Spectrometry Software Waters N/A SigmaPlot 12.3 Systat Software systatsoftware.com Newbler V2.7 454 Life Sciences www.454.com Phred, Phrap, and Consed software packages Laboratory of PHIL GREEN http://www.phrap.org/ phredphrapconsed.html tRNAscan-SE 1.3.1 Lowe and Eddy, 1997 N/A CRISPRFinder Grissa et al., 2007 N/A Prodigal-2.6 Hyatt et al., 2010 N/A Tophat v2.1.0 Trapnell et al., 2009 N/A Cufflinks v2.2.1 Trapnell et al., 2010; Roberts et al., 2011 N/A e1 Cell Chemical Biology 24, 1–13.e1–e7, June 22, 2017 ..
Recombinant:Article Title: Biochemical Mechanisms and Catabolic Enzymes Involved in Bacterial Estrogen Degradation Pathways.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains Sphingomonas sp. strain KC8 Yu et al., 2007 N/A Escherichia coli BL21 (DE3) Yeastern Biotech Cat# FYE207-40VL Escherichia coli One Shot TOP10 Invitrogen Cat# C404010 Chemicals, Peptides, and Recombinant Proteins 17b-Estradiol (E2) Sigma-Aldrich Cat# E8875 Estrone (E1) Sigma-Aldrich Cat# E9750 4-Hydroxyestrone Sigma-Aldrich Cat# H6012 Dimethyl sulfoxide Sigma-Aldrich Cat# 34943 Testosterone Sigma-Aldrich Cat# 86500 Oxygen-18O2 (97 atom %) Sigma-Aldrich Cat# 490474 Estrone (3,4-13C2, 99%) Cambridge Isotope Laboratories Cat# CLM-673 Testosterone (2,3,4-13C3, 98%) Isosciences Cat# 6066 Pyridinestrone acid This paper N/A OecAhis This paper N/A OecChis This paper N/A Critical Commercial Assays XenoScreen YES/YAS Assay Xenometrix Cat# NO5-230-P In-Fusion HD Cloning Kit Clontech Cat# 639649 pEXP-5-NT/TOPO TA Expression Kit Invitrogen Cat# V96005 Deposited Data Genome sequence of Spingomonas sp. strain KC8 This paper NCBI: CP016306 Oligonucleotides pET21b-oecA forward primer: 50-GAAGGAGATATACATATGGATGGCGATCAATCG-30 This paper N/A pET21b-oecA reverse primer: 50-GTGGTGGTGCTCGAGGCCCTGCTTCGGCG-30 This paper N/A oecC forward primer (oecC_for): 50-TTGAAGATCTGCGGCTTGGGCT-30 This paper N/A oecC reverse primer (oecC_rev): 50-TCAGCCTGCGGCCACATCGTCGGC-30 This paper N/A Recombinant DNA pET21b Novagen Cat# 69741 pET21b-oecA This paper N/A pEXP-5-NT/TOPO-oecC This paper N/A Software and Algorithms MassLynx Mass Spectrometry Software Waters N/A SigmaPlot 12.3 Systat Software systatsoftware.com Newbler V2.7 454 Life Sciences www.454.com Phred, Phrap, and Consed software packages Laboratory of PHIL GREEN http://www.phrap.org/ phredphrapconsed.html tRNAscan-SE 1.3.1 Lowe and Eddy, 1997 N/A CRISPRFinder Grissa et al., 2007 N/A Prodigal-2.6 Hyatt et al., 2010 N/A Tophat v2.1.0 Trapnell et al., 2009 N/A Cufflinks v2.2.1 Trapnell et al., 2010; Roberts et al., 2011 N/A e1 Cell Chemical Biology 24, 1–13.e1–e7, June 22, 2017 ..
Cloning:Article Title: Biochemical Mechanisms and Catabolic Enzymes Involved in Bacterial Estrogen Degradation Pathways.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains Sphingomonas sp. strain KC8 Yu et al., 2007 N/A Escherichia coli BL21 (DE3) Yeastern Biotech Cat# FYE207-40VL Escherichia coli One Shot TOP10 Invitrogen Cat# C404010 Chemicals, Peptides, and Recombinant Proteins 17b-Estradiol (E2) Sigma-Aldrich Cat# E8875 Estrone (E1) Sigma-Aldrich Cat# E9750 4-Hydroxyestrone Sigma-Aldrich Cat# H6012 Dimethyl sulfoxide Sigma-Aldrich Cat# 34943 Testosterone Sigma-Aldrich Cat# 86500 Oxygen-18O2 (97 atom %) Sigma-Aldrich Cat# 490474 Estrone (3,4-13C2, 99%) Cambridge Isotope Laboratories Cat# CLM-673 Testosterone (2,3,4-13C3, 98%) Isosciences Cat# 6066 Pyridinestrone acid This paper N/A OecAhis This paper N/A OecChis This paper N/A Critical Commercial Assays XenoScreen YES/YAS Assay Xenometrix Cat# NO5-230-P In-Fusion HD Cloning Kit Clontech Cat# 639649 pEXP-5-NT/TOPO TA Expression Kit Invitrogen Cat# V96005 Deposited Data Genome sequence of Spingomonas sp. strain KC8 This paper NCBI: CP016306 Oligonucleotides pET21b-oecA forward primer: 50-GAAGGAGATATACATATGGATGGCGATCAATCG-30 This paper N/A pET21b-oecA reverse primer: 50-GTGGTGGTGCTCGAGGCCCTGCTTCGGCG-30 This paper N/A oecC forward primer (oecC_for): 50-TTGAAGATCTGCGGCTTGGGCT-30 This paper N/A oecC reverse primer (oecC_rev): 50-TCAGCCTGCGGCCACATCGTCGGC-30 This paper N/A Recombinant DNA pET21b Novagen Cat# 69741 pET21b-oecA This paper N/A pEXP-5-NT/TOPO-oecC This paper N/A Software and Algorithms MassLynx Mass Spectrometry Software Waters N/A SigmaPlot 12.3 Systat Software systatsoftware.com Newbler V2.7 454 Life Sciences www.454.com Phred, Phrap, and Consed software packages Laboratory of PHIL GREEN http://www.phrap.org/ phredphrapconsed.html tRNAscan-SE 1.3.1 Lowe and Eddy, 1997 N/A CRISPRFinder Grissa et al., 2007 N/A Prodigal-2.6 Hyatt et al., 2010 N/A Tophat v2.1.0 Trapnell et al., 2009 N/A Cufflinks v2.2.1 Trapnell et al., 2010; Roberts et al., 2011 N/A e1 Cell Chemical Biology 24, 1–13.e1–e7, June 22, 2017 ..
Expressing:Article Title: Biochemical Mechanisms and Catabolic Enzymes Involved in Bacterial Estrogen Degradation Pathways.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains Sphingomonas sp. strain KC8 Yu et al., 2007 N/A Escherichia coli BL21 (DE3) Yeastern Biotech Cat# FYE207-40VL Escherichia coli One Shot TOP10 Invitrogen Cat# C404010 Chemicals, Peptides, and Recombinant Proteins 17b-Estradiol (E2) Sigma-Aldrich Cat# E8875 Estrone (E1) Sigma-Aldrich Cat# E9750 4-Hydroxyestrone Sigma-Aldrich Cat# H6012 Dimethyl sulfoxide Sigma-Aldrich Cat# 34943 Testosterone Sigma-Aldrich Cat# 86500 Oxygen-18O2 (97 atom %) Sigma-Aldrich Cat# 490474 Estrone (3,4-13C2, 99%) Cambridge Isotope Laboratories Cat# CLM-673 Testosterone (2,3,4-13C3, 98%) Isosciences Cat# 6066 Pyridinestrone acid This paper N/A OecAhis This paper N/A OecChis This paper N/A Critical Commercial Assays XenoScreen YES/YAS Assay Xenometrix Cat# NO5-230-P In-Fusion HD Cloning Kit Clontech Cat# 639649 pEXP-5-NT/TOPO TA Expression Kit Invitrogen Cat# V96005 Deposited Data Genome sequence of Spingomonas sp. strain KC8 This paper NCBI: CP016306 Oligonucleotides pET21b-oecA forward primer: 50-GAAGGAGATATACATATGGATGGCGATCAATCG-30 This paper N/A pET21b-oecA reverse primer: 50-GTGGTGGTGCTCGAGGCCCTGCTTCGGCG-30 This paper N/A oecC forward primer (oecC_for): 50-TTGAAGATCTGCGGCTTGGGCT-30 This paper N/A oecC reverse primer (oecC_rev): 50-TCAGCCTGCGGCCACATCGTCGGC-30 This paper N/A Recombinant DNA pET21b Novagen Cat# 69741 pET21b-oecA This paper N/A pEXP-5-NT/TOPO-oecC This paper N/A Software and Algorithms MassLynx Mass Spectrometry Software Waters N/A SigmaPlot 12.3 Systat Software systatsoftware.com Newbler V2.7 454 Life Sciences www.454.com Phred, Phrap, and Consed software packages Laboratory of PHIL GREEN http://www.phrap.org/ phredphrapconsed.html tRNAscan-SE 1.3.1 Lowe and Eddy, 1997 N/A CRISPRFinder Grissa et al., 2007 N/A Prodigal-2.6 Hyatt et al., 2010 N/A Tophat v2.1.0 Trapnell et al., 2009 N/A Cufflinks v2.2.1 Trapnell et al., 2010; Roberts et al., 2011 N/A e1 Cell Chemical Biology 24, 1–13.e1–e7, June 22, 2017 ..
Sequencing:Article Title: Biochemical Mechanisms and Catabolic Enzymes Involved in Bacterial Estrogen Degradation Pathways.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains Sphingomonas sp. strain KC8 Yu et al., 2007 N/A Escherichia coli BL21 (DE3) Yeastern Biotech Cat# FYE207-40VL Escherichia coli One Shot TOP10 Invitrogen Cat# C404010 Chemicals, Peptides, and Recombinant Proteins 17b-Estradiol (E2) Sigma-Aldrich Cat# E8875 Estrone (E1) Sigma-Aldrich Cat# E9750 4-Hydroxyestrone Sigma-Aldrich Cat# H6012 Dimethyl sulfoxide Sigma-Aldrich Cat# 34943 Testosterone Sigma-Aldrich Cat# 86500 Oxygen-18O2 (97 atom %) Sigma-Aldrich Cat# 490474 Estrone (3,4-13C2, 99%) Cambridge Isotope Laboratories Cat# CLM-673 Testosterone (2,3,4-13C3, 98%) Isosciences Cat# 6066 Pyridinestrone acid This paper N/A OecAhis This paper N/A OecChis This paper N/A Critical Commercial Assays XenoScreen YES/YAS Assay Xenometrix Cat# NO5-230-P In-Fusion HD Cloning Kit Clontech Cat# 639649 pEXP-5-NT/TOPO TA Expression Kit Invitrogen Cat# V96005 Deposited Data Genome sequence of Spingomonas sp. strain KC8 This paper NCBI: CP016306 Oligonucleotides pET21b-oecA forward primer: 50-GAAGGAGATATACATATGGATGGCGATCAATCG-30 This paper N/A pET21b-oecA reverse primer: 50-GTGGTGGTGCTCGAGGCCCTGCTTCGGCG-30 This paper N/A oecC forward primer (oecC_for): 50-TTGAAGATCTGCGGCTTGGGCT-30 This paper N/A oecC reverse primer (oecC_rev): 50-TCAGCCTGCGGCCACATCGTCGGC-30 This paper N/A Recombinant DNA pET21b Novagen Cat# 69741 pET21b-oecA This paper N/A pEXP-5-NT/TOPO-oecC This paper N/A Software and Algorithms MassLynx Mass Spectrometry Software Waters N/A SigmaPlot 12.3 Systat Software systatsoftware.com Newbler V2.7 454 Life Sciences www.454.com Phred, Phrap, and Consed software packages Laboratory of PHIL GREEN http://www.phrap.org/ phredphrapconsed.html tRNAscan-SE 1.3.1 Lowe and Eddy, 1997 N/A CRISPRFinder Grissa et al., 2007 N/A Prodigal-2.6 Hyatt et al., 2010 N/A Tophat v2.1.0 Trapnell et al., 2009 N/A Cufflinks v2.2.1 Trapnell et al., 2010; Roberts et al., 2011 N/A e1 Cell Chemical Biology 24, 1–13.e1–e7, June 22, 2017 ..
Software:Article Title: Biochemical Mechanisms and Catabolic Enzymes Involved in Bacterial Estrogen Degradation Pathways.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains Sphingomonas sp. strain KC8 Yu et al., 2007 N/A Escherichia coli BL21 (DE3) Yeastern Biotech Cat# FYE207-40VL Escherichia coli One Shot TOP10 Invitrogen Cat# C404010 Chemicals, Peptides, and Recombinant Proteins 17b-Estradiol (E2) Sigma-Aldrich Cat# E8875 Estrone (E1) Sigma-Aldrich Cat# E9750 4-Hydroxyestrone Sigma-Aldrich Cat# H6012 Dimethyl sulfoxide Sigma-Aldrich Cat# 34943 Testosterone Sigma-Aldrich Cat# 86500 Oxygen-18O2 (97 atom %) Sigma-Aldrich Cat# 490474 Estrone (3,4-13C2, 99%) Cambridge Isotope Laboratories Cat# CLM-673 Testosterone (2,3,4-13C3, 98%) Isosciences Cat# 6066 Pyridinestrone acid This paper N/A OecAhis This paper N/A OecChis This paper N/A Critical Commercial Assays XenoScreen YES/YAS Assay Xenometrix Cat# NO5-230-P In-Fusion HD Cloning Kit Clontech Cat# 639649 pEXP-5-NT/TOPO TA Expression Kit Invitrogen Cat# V96005 Deposited Data Genome sequence of Spingomonas sp. strain KC8 This paper NCBI: CP016306 Oligonucleotides pET21b-oecA forward primer: 50-GAAGGAGATATACATATGGATGGCGATCAATCG-30 This paper N/A pET21b-oecA reverse primer: 50-GTGGTGGTGCTCGAGGCCCTGCTTCGGCG-30 This paper N/A oecC forward primer (oecC_for): 50-TTGAAGATCTGCGGCTTGGGCT-30 This paper N/A oecC reverse primer (oecC_rev): 50-TCAGCCTGCGGCCACATCGTCGGC-30 This paper N/A Recombinant DNA pET21b Novagen Cat# 69741 pET21b-oecA This paper N/A pEXP-5-NT/TOPO-oecC This paper N/A Software and Algorithms MassLynx Mass Spectrometry Software Waters N/A SigmaPlot 12.3 Systat Software systatsoftware.com Newbler V2.7 454 Life Sciences www.454.com Phred, Phrap, and Consed software packages Laboratory of PHIL GREEN http://www.phrap.org/ phredphrapconsed.html tRNAscan-SE 1.3.1 Lowe and Eddy, 1997 N/A CRISPRFinder Grissa et al., 2007 N/A Prodigal-2.6 Hyatt et al., 2010 N/A Tophat v2.1.0 Trapnell et al., 2009 N/A Cufflinks v2.2.1 Trapnell et al., 2010; Roberts et al., 2011 N/A e1 Cell Chemical Biology 24, 1–13.e1–e7, June 22, 2017 ..
Mass Spectrometry:Article Title: Biochemical Mechanisms and Catabolic Enzymes Involved in Bacterial Estrogen Degradation Pathways.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains Sphingomonas sp. strain KC8 Yu et al., 2007 N/A Escherichia coli BL21 (DE3) Yeastern Biotech Cat# FYE207-40VL Escherichia coli One Shot TOP10 Invitrogen Cat# C404010 Chemicals, Peptides, and Recombinant Proteins 17b-Estradiol (E2) Sigma-Aldrich Cat# E8875 Estrone (E1) Sigma-Aldrich Cat# E9750 4-Hydroxyestrone Sigma-Aldrich Cat# H6012 Dimethyl sulfoxide Sigma-Aldrich Cat# 34943 Testosterone Sigma-Aldrich Cat# 86500 Oxygen-18O2 (97 atom %) Sigma-Aldrich Cat# 490474 Estrone (3,4-13C2, 99%) Cambridge Isotope Laboratories Cat# CLM-673 Testosterone (2,3,4-13C3, 98%) Isosciences Cat# 6066 Pyridinestrone acid This paper N/A OecAhis This paper N/A OecChis This paper N/A Critical Commercial Assays XenoScreen YES/YAS Assay Xenometrix Cat# NO5-230-P In-Fusion HD Cloning Kit Clontech Cat# 639649 pEXP-5-NT/TOPO TA Expression Kit Invitrogen Cat# V96005 Deposited Data Genome sequence of Spingomonas sp. strain KC8 This paper NCBI: CP016306 Oligonucleotides pET21b-oecA forward primer: 50-GAAGGAGATATACATATGGATGGCGATCAATCG-30 This paper N/A pET21b-oecA reverse primer: 50-GTGGTGGTGCTCGAGGCCCTGCTTCGGCG-30 This paper N/A oecC forward primer (oecC_for): 50-TTGAAGATCTGCGGCTTGGGCT-30 This paper N/A oecC reverse primer (oecC_rev): 50-TCAGCCTGCGGCCACATCGTCGGC-30 This paper N/A Recombinant DNA pET21b Novagen Cat# 69741 pET21b-oecA This paper N/A pEXP-5-NT/TOPO-oecC This paper N/A Software and Algorithms MassLynx Mass Spectrometry Software Waters N/A SigmaPlot 12.3 Systat Software systatsoftware.com Newbler V2.7 454 Life Sciences www.454.com Phred, Phrap, and Consed software packages Laboratory of PHIL GREEN http://www.phrap.org/ phredphrapconsed.html tRNAscan-SE 1.3.1 Lowe and Eddy, 1997 N/A CRISPRFinder Grissa et al., 2007 N/A Prodigal-2.6 Hyatt et al., 2010 N/A Tophat v2.1.0 Trapnell et al., 2009 N/A Cufflinks v2.2.1 Trapnell et al., 2010; Roberts et al., 2011 N/A e1 Cell Chemical Biology 24, 1–13.e1–e7, June 22, 2017 ..
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